Synthetic Biology

Synthetic Biology

Synthetic Biology Software

AgroNT: tool that analyzes plant DNA sequences to predict gene modifications

BindNet: protein-ligand binding representation learning from fine-grained interactions

BioNetGen: structure-based modeling of biochemical reaction networks

BioCRNplyer: biomolecular chemical reaction network compiler

BioGRID: large database of biological interactions modeled at the molecular level (proteins, genes, chemicals), not at the atomic bond level

BioNetGen: rule based biochemical reaction network

Cello: genetic circuit design using logic gates

COPASI: modeling biochemical reaction networks

COBRA: whole-cell metabolic modeler that you can call as a package (E. Coli, etc)

DNA Chisel: codon optimization and solving sequence constraints

EC-KitY: python toolkit for evolutionary computation that integrates with sci-kit learn

GapMind: maps out carbon utilization pathways for a genome. You can compare how complete each pathway is across your organism and see which are missing entirely in an organism

iBioSim: genetic circuit simulation and analysis

KBase: AWS for systems bio analysis, hosted by the Department of Energy

PoSeiDon: a pipeline to detect significantly positive selected sites and possible recombination events in an alignment of multiple protein-coding sequences

PySB: systems biology modeling in Python

RecombineX: genome engineering toolkit

RBS Calculator: directly outputs a DNA sequence based on a need- if a specific amount of protein is needed, the tool will suggest an RBS design that is accurate within a variance factor of 2.3 (turns this into a reliable tool)

SBML: standardized models for systems biology as a markup language, use it as a data format to describe synthetic biology code

SBOL canvas: genetic circuit schematic building using SBOL

SnapGene: visual plasmid construction + simulation

SpliceVarDB: variant database for splicing

TCellMatch: collection of models to predict which antigens a T-cell receptor will bind, based on single-cell sequence data; test immune design before lab work

TISIGNER-ReactJS: pathway optimization in E Coli

Uniprot/PDB: protein sequence + 3D structure databases

Virtual Metabolic Human: represent metabolic diagram on a chip

SynthBio Tools

Biometra TRobot: PCR machine

Digital to Biological Converter (DBC): a standalone machine that converts any input sequence to its chemical counterpart- DNA, RNA, proteins, viruses (1 → many type converter)

FlowJo: flow cytometry data analysis software (1996)

Gibson Assembly: method for joining/stitching DNA together

JCat: codon optimization tool

PUREexpress: cell free expression system

Sleeping Beauty (SB100): transposase system for stable circuit integration in mouse liver cancer models

SOLO Automated Liquid Handler: high resolution, compact robotic pipettor